Prof. Roger M. LeblancProfessor and Chair
Department of Chemistry, University of Miami, USA
Speech Title: Carbon Dots as Versatile Drug Nanocarriers in Modern Medicine
Abstract: Carbon dots (CDs) with size less than 10 nm have recently triggered great attention in the research of material science and biomedical engineering due to their unique properties such as small size, excellent photoluminescence (PL), high water-dispersity, biocompatibility, nontoxicity and abundant surface functionalities. In this presentation, I will firstly introduce diverse preparations of CDs. Extensive structural characterizations have been used to hypothesize comprehensive structural models for 3 distinct CD species that represent both top-down and bottom-up approaches in order to optimize their properties and applications.
Then, I will mainly focus on many excellent biomedical applications of the CDs recently developed in our lab: (1), in vivo experiment suggested that glucose-based CDs could cross the blood-brain barrier (BBB) due to the presence of glucose transporter proteins on the BBB; (2), a drug delivery system of carbon nitride dots conjugated with an anti-cancer therapeutic drug and a targeting molecule was capable of effective treatment against diffuse large B-cell lymphoma both in vitro and in vivo revealing efficient therapeutic capabilities with minimal toxic side effects; (3), metformin-derived CDs showed a unique nucleus targeting property, which suggests a huge potential for future nucleus-targeting drug delivery; (4), CDs have constantly shown the capability to inhibit the formation of amyloid precursor protein (APP), beta-amyloid (Aβ) and Aβ fibrils. CDs are promising nanomedicine and drug nanocarriers to treat Alzheimer’s disease (AD); (5) a pilot study showed a versatile nanocarrier could be assembled via the direct conjugation between distinct CDs to fulfill multitasks.
Keywords: carbon dots, nanocarrier, blood-brain barrier, drug delivery, cancer treatment, Alzheimer’s disease, nanoparticle assembly
Biography: Roger M. Leblanc received his B. S. in chemistry in 1964 from Université Laval, Canada, and Ph. D. in physical chemistry in1968 from the same university. From 1968 to 1970, he was a postdoctoral fellow in the laboratory of Prof. George Porter, FRS, in Davy Faraday Research Lab, the Royal Institution of Great Britain. He was a professor from 1970 to 1993 at Department of Chemistry and Biology in Université du Québec à Trois Rivières, Canada. During this period, he was Chair from 1971 to 1975 at the same department, and Director from 1981 to 1991 at Photobiophysics Research Center. In 1994, he moved to University of Miami, where he has been a professor at Department of Chemistry since then. At University of Miami, he was Chair of Department of Chemistry from 1994 to 2002, and he is reappointed as Chair from 2013 to present. He is also Professor (Second Appointment) of the Department of Dermatology and Cutaneous Surgery, Miller School of Medicine, University of Miami (Miami, Florida, USA) and Department of Physics, University of Miami (Coral Gables, Florida, USA). In addition, he is Senior Fellow of the University of Miami Center on Aging (COA) (Coral Gables, Florida, USA).
Dr. Leblanc has very rich experiences in materials chemistry, especially in the area of preparation of new materials and their bio applications development. He also has a strong background in physical chemistry, with a sound expertise in key research areas for this application, in particular in surface chemistry, microscopy (Brewster Angle microscopy, epi-fluorescence imaging, atomic force microscopy, and transmission electron microscopy) and spectroscopy (UV/vis, fluorescence, and infrared reflectionabsorption spectroscopy). Dr. Leblanc’s contributions to materials chemistry and surface chemistry have earned him domestic and international recognitions: In 1987, he received the University Merit Medal for excellent academic activities from University of Québec, Trois-Rivières, Québec, Canada; In 1992, he won the John-Labatt Limited Award of the Canadian Society for Chemistry for outstanding achievement in chemical research with particular emphasis on biological systems; In the same year, he was appointed as the Honorary Professor of Jilin University, Jilin, Changchun, China; In 1993, he obtained the Governor General Medal in recognition of significant contribution to compatriots, community and to Canada and there was a scholarship established in his name (for graduate students in Biophysics, University of Québec) in recognition of exceptional contribution for development of graduate studies; In 1997, he received a plaque in recognition and appreciation for contributions to the Chemistry Department as Chairman from University of Miami; In 1999, he was presented with an award for exemplary scientific advancements and department leadership by College of Arts and Sciences and Department of Chemistry at University of Miami; In 2002, he won the Provost’s Award for scholarly activity and was invited to become the honorary member of the Societe Royale des Sciences de Liege, Belgium, and the Society of Physical Chemists of Serbia for his contribution to the development of Physical Chemistry; In 2006, he got the 2006 Florida Award, American Chemical Society in recognition of the significant and meritorious achievements of prominent Florida Scientists in 2006; In 2011, Dr. Leblanc obtained Cooper Fellowship in recognition of the excellence in contributing to the core missions of scholarship, teaching and service at the College of Arts & Sciences (University of Miami); in 2014, he won the “Faculty Mentor of the Year” Award (University of Miami); in 2019, he received the “Journal of Oleo Science Impact Award” in recognition of the manuscript published in the Journal of Oleo Science most cited as a reference in other journals during the year.
The innovative work of Dr. Leblanc’s multidisciplinary team has long focused on the development of novel nanomaterials such as carbon dots. After synthesis, purification and characterization by various spectroscopies and microscopies, we are especially interested in the application of carbon dots in drug delivery to treat Alzheimer’s disease, bone mineralization process and many types of cancer. Except for drug delivery, our research group is also investigating the application of 2D or 3D printing, biosensing, photocatalysis and cosmetics using carbon dots. Another focus of our research group is the study of surface chemistry of organic and biological supramolecular complexes using Langmuir monolayer technique.
Dr. Leblanc has published 534 scientific articles in peer-reviewed journals, and he has given more than 430 presentations at medical and scientific conferences around the world. He is guest editor of Molecules. He is on the Editorial Advisory Board of several professional journals including Colloids and Surfaces B: Biointerfaces, Journal of Parkinson’s Disease and Alzheimer's Disease, Analytical and Bioanalytical Chemistry, The Open Inorganic Chemistry Journal and Recent Patents on Material Science. He is also past editor (or guest editor) of Canadian Journal of Botany, Colloids and Surfaces B: Biointerfaces, Polymers and Journal of Biological Systems.
Dr. Leblanc was a member of the International Advisory Committee (IAC) for the 15th Surfactants in Solution (SIS) Symposium (Fortaleza, Brazil, June 6-11, 2004) and Scientific and Technical Program Committee for the 13th International Symposium on Surfactants in Solution (SIS-2000) (Gainesville, FL, University of Florida, June 11-16, 2000). And he was an organizer of the Symposium on Self-Assembly and Langmuir-Blodgett Film Technology for Chemi- and Biosensors during the Southeast Regional Meeting of the American Chemical Society (SERMACS - 99) (Hyatt Regency Hotel, Knoxville, TN, October 17-20, 1999). In addition, he was the Vice Chairman of Organizing Committee for the Bionic Design International Workshop: Reconstruction of Biological Functions and Structures (Tsukuba, Japan, January 29-30, 1992).
Prof. Nongyue HeState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, P.R. China
Speech Title: Development and Application of a Novel Sample In–Answer Out (SIAO) System Based on a cartridge and Magnetic Nanoparticles
Abstract: Because it has many advantages such as rapidity and accuracy, nucleic acid detection is applied for infectious disease diagnosis more and more. On the other hand, the prevention, control, diagnosis and treatment of infectious diseases have become a global focus for public health. While the traditional methods for pathogen testing have some major disadvantages including the need for highly skilled staff and expensive instrumentation, while procedural aspects are complex and sensitive to the environment. These shortcomings have greatly limited the application of traditional testing in on site pathogen detection. In recent years, an automatic integrated nucleic acid detection system based on real-time PCR is developed by our research group to conduct point-of-care testing of infectious pathogens. The home-made detection system collects fluorescence data in each PCR cycle through an integrated dual-channel fluorescence detection module and then real-time fluorescence curves are drawn by the software, which can tell the results of the diagnostics after some processing and analysis. In this paper, we present a new point-of-care-testing (POCT) system based on magnetic nanoparticles that enable sample in–answer out (SIAO) automated real-time testing for pathogens. Various performance tests were conducted on the instrument. Nucleic acid extraction efficiencies of SIAO versus manual systems were 95.49% and 84.33%, respectively. Real-time PCR by two methods (TaqMan-based probe and SYBR green dye) in the SIAO system was achievable, with comparable results to the manual method. Nucleic acid testing with the SIAO system was repeatable and better than with manual testing. The SIAO system had good anti-pollution performance with easy avoidance of inter-assay cross contamination. Finally, use of the SIAO system for adenovirus detection produced similar results to LightCycler2.0 system assay findings. The amplification plots and Ct values suggested similar amplification plots shapes for adenovirus testing with the SIAO system and with real-time fluorescence PCR testing and commercial instrument post-manual nucleic acid extraction. Collectively, these findings indicate that testing with the SIAO system is virtually equivalent to that of manual extraction with commercial system testing.
Biography: Nongyue He completed his PhD degree from Nanjing University, China. Initially, he joined Southeast University, Nanjing and Nanjing Medical University, Nanjing as a postdoctoral researcher. But since 2000, he emerged as a professor in State Key Laboratory of Bioelectronics, School of Biomedical Science and Medical Engineering, Southeast University, Nanjing. His research interests are focused on biochips and biosensors, functional nanomaterials, controlled drug release and tissue engineering. He gained several awards like the Award for Excellent Teachers in Chinese Universities (2002), the Second Grade Award for Natural Science (2015), the Second Grade Award for Advance in Science and Technology (2014) of Chinese Ministry of Education; the Second Grade Award for Natural Science (2010, 2016), the Third Grade Award for Advance in Science and Technology (2007), and the First Grade Award for Technology Invention (2013) of Hunan Province; Chien-Shiung Wu Award (2002) of Southeast University; the First Grade Award for Advance in Science and Technology (2017) of Nanjing City, Jiang; and the First Grade Award of Endoscopic Medical Tech-Science in China (2008). He has published more than 300 scientific journal articles, 6 books, and more than 55 patents.